chromosome walking

An Improved Method of Chromosome Walking for Promoter Sequences Cloning

一种改良的启动子序列克隆的染色体步查法

Chromosome walking techniques are commonly used for cloning the known flanking sequence .

染色体步行是一种常用的克隆已知基因旁侧序列的技术。

Various PCR-based methods are available for chromosome walking from a known sequence to an unknown region .

的方法能够根据已知的基因序列得到侧翼的基因序列。

PCR Techniques for Chromosome Walking

染色体步行PCR技术

Construction of a YAC Contig Encompassing G200 Locus via Chromosome Walking

通过染色体步查建立水稻G200座位的重叠群

Single primer PCR was a method of chromosome walking to isolate sequences flanking a known DNA sequence with only one primer .

单引物PCR是一种只用一条引物就可以克隆已知序列侧翼未知区域的染色体步移方法。

A novel technique , isocaudarners inverse PCR ( II-PCR ), has been established for chromosome walking .

本研究在反向PCR基础上，建立了一种新型染色体步移技术：同尾酶反向PCR。

The main results are as follows in the study : Using the methods of degenerate PCR and chromosome walking , this paper obtained the full sequence information of ACC gene from Rhodotorula glutinis for the first time .

本研究主要结果如下：利用简并PCR和染色体步移法首次克隆获得产油粘红酵母（Rhodotorulaglutinis）ACC基因的全长序列信息。

Started with ACO1 ( aconitase I ), we got positive clone of ACO1 then obtained 16 clones by chromosome walking . After analysis fingerprinting of 16 BAC clones .

以离ID最近的ACO1（aconitase1，顺乌头酸酶）基因为起点，设计引物筛选含ACO1的BAC克隆，用染色体步行的方法得到16个BAC克隆。

This paper summarized the main processes of the map-based cloning including preliminary mapping , fine scale-mapping of candidate gene , building genetic map , chromosome walking or landing and finally function complement experiment to identify candidate gene .

本文主要概述了图位克隆的一般步骤，包括目的基因的初步定位、精细定位和遗传做图、染色体步行和登陆及利用功能互补实验鉴定目的基因。

Using the homologous cloning strategy with degenerate primers and chromosome walking techniques , two genes fragments encoding cellulase were cloned from one bacterial strain Rhizobium radiobacter isolated from the gut of H. parallela larvae .

本研究使用兼并引物同源克隆并结合染色体步移的方法，从1株暗黑鳃金龟肠道内的纤维素降解菌Rhizobiumradiobacter基因组内成功获得2个纤维素酶编码基因。

An ortholog of CBF : ApCBF ( DQ207404 ) was amplified and cloned by PCR and chromosome walking from the genomic DNA of the Arabidopsis pumila . It is special in Xinjiang .

利用PCR及染色体步移法从新疆特有拟南芥近源种植物&小拟南芥的基因组中克隆了CBF基因的同源序列（DQ207404）。